Novel pilot bioreactor for scale up Gynura procumbens adventitious root culture

Adventitious root culture and the development of a bioreactor are methods to obtain biomass plant bioactive compounds in large quantities faster than conventional cultivation. These technologies provide an excellent opportunity produce from plants, especially Gynura procumbens. Previous reports mentioned small-scale could increase G. procumbens adventitious roots. Therefore, larger for is necessary develop. In this study, 19 L pilot was successful. The optimal condition sterilization 1.8 bar 60 min using autoclave. We found that roots bioreactors has resulted MS-Tek media (technical grade) compared MS-PA (pro analyze) after 35 d period. Although, higher productivity total phenolics flavonoids been achieved rather media. further it evaluate effect technical-grade chemicals on kinetics growth chemical uptake. hope suitable formulation with affordable cost production.